Volume : 3, Issue : 4, April - 2014

Identification of Secondary Metabolites Present in Carica Papaya L. Leaf Extract Found In Northern India by Hptlc & Other Test Procedures, its Significance and Role in Curing Dengue/DHF Disease

Dinesh Kumar Sharma, Prof. B. Tiwari

Abstract :

The present study was performed for the development of physico–chemical parameters of Carica papaya Linn. Leaves (CP) belong to family Caricaceae. The study comprises physico–chemical and phytochemical evaluation to confirm the Secondary metabolites present in Carica papaya leaf. The TLC fingerprinting profile of different extracts developed which exhibited the presence of several secondary metabolites. The methods adopted here for the identification of Secondary Metabolites is simple, specific, accurate and rapid also. Extraction from the plant is an empirical exercise in which different solvents are utilized under a variety of conditions such as time and temperature during extraction. After extraction, the bioactive components were separated from the co extractives. Further steps for the isolation involved is the solvent partition of the co extractives or chromatographic methods in order to fractionate the compounds based on their acidity, polarity or molecular size and Qualitative methods also. For purification some structural analysis, may be accomplished by appropriate techniques such as recrystallisation, sublimation, distillation/extraction. Here, the distillation process was done. The main purpose for this study is to find out the role of phytochemicals, particularly secondary metabolites present in Carica papaya L. Leaves in curing the disease Dengue/DHF directly or indirectly.