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MENUVolume : 4, Issue : 4, April - 2015
Listeria monocytogenes biofilms on food contact surfaces under different growth conditions
K Vrinda Menon, B Sunil, C Latha, Nithya K. R, Ashok Kumar
Abstract :
<p><p>&lt;p&gt;&amp;nbsp;Listeria monocytogenes is an opportunistic intracellular pathogen that has become an important cause of foodborne&amp;nbsp;&lt;/p&gt; &lt;div&gt;infections worldwide. The organism possesses an ability to form biofilms on food processing surfaces, potentially&amp;nbsp;&lt;/div&gt; &lt;div&gt;leading to food product contamination. In the present study, stainless steel (SS) and cutting board fiber (FIB) coupons&amp;nbsp;&lt;/div&gt; &lt;div&gt;were used as the substratum for biofilm growth in the presence of sterile distilled water and Tryptone soya oth (TSB) as nutrient medium. The&amp;nbsp;&lt;/div&gt; &lt;div&gt;Listeria monocytogenes standard culture (MTCC 1143) belonging to serotype 4b at a concentration of 6 log 10 cfu/ ml was used for creating&amp;nbsp;&lt;/div&gt; &lt;div&gt;biofilms on these surfaces. The formation of biofilms was assessed after 24 hours of incubation at 370 C. The formation of biofilms on these&amp;nbsp;&lt;/div&gt; &lt;div&gt;surfaces after 24 hrs was observed by scanning electron microscopy (SEM) which revealed the presence of biofilms on both surfaces with more&amp;nbsp;&lt;/div&gt; &lt;div&gt;pronounced biofilm formation on fiber as compared to stainless steel in the presence of TSB as nutrient medium. The presence of irreversible&amp;nbsp;&lt;/div&gt; &lt;div&gt;biofilms with TSB as nutrient medium after washing the coupons was assessed which revealed the recovery of the organism at the level of 3.77 &amp;plusmn;&amp;nbsp;&lt;/div&gt; &lt;div&gt;0.13 log 10 cfu/cm2 and 4.91&amp;plusmn; 0.33 log 10 cfu/cm2 on SS and FIB surfaces respectively. The presence of sterile distilled water as nutrient medium&amp;nbsp;&lt;/div&gt; &lt;div&gt;also revealed the recovery of the organisms but were at the level of 1.82 &amp;plusmn; 0.18 log 10 cfu/cm2 and 2.80 &amp;plusmn; 0.13 log 10 cfu/cm2 on SS and FIB&amp;nbsp;&lt;/div&gt; &lt;div&gt;surfaces respectively. The formation of biofilms even in the presence of sterile distilled water is an area of great concern for the food industry.&amp;nbsp;&lt;/div&gt; &lt;div&gt;However the study revealed that the difference in the characteristics of surfaces revealed a difference in the ability of the organism to form biofilm&amp;nbsp;&lt;/div&gt; &lt;div&gt;as the biofilm was more pronounced on the fiber as compared to stainless steel. Hence, biofilm growth of L. monocytogenes was sufficient to&amp;nbsp;&lt;/div&gt; &lt;div&gt;provide a substantial risk of this pathogen contaminating the food&amp;ndash;processing plant environment if wet surfaces are not maintained in a sanitary&amp;nbsp;&lt;/div&gt; &lt;div&gt;condition.&lt;/div&gt;</p></p>
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Download PDF Journal DOI : 10.15373/2249555XCite This Article:
K VRINDA MENON, B SUNIL, C LATHA, NITHYA K.R, ASHOK KUMAR Listeria monocytogenes biofilms on food contact surfaces under different growth conditions Global Journal For Research Analysis, Vol: 4, Issue: 4 April 2015
