Volume : 5, Issue : 9, September - 2016

Detection and Genomic Characterization of Carbapenemase producing Pseudomonas aeruginosaisolates in Chronic SuppurativeOtitis Media(CSOM)

Dr. D. Neelaveni, Dr. P. Sankar

Abstract :

<p>&nbsp;Introduction: Chronic Suppurative Otitis Media is a common infection in the middle ear and an important cause ofhearing impairment in developing countries. Aims and Objectives: To determine the Carbapenemase producing Multi drug resistant (MDR) Pseudomonas aeruginosa isolates in CSOM. Materials and Methods: 140 ear swabs were collected from 130 patients with CSOM. The isolates were identified and tested for antimicrobial susceptibility.Pseudomonas isolates with MIC &gt;32 were selected for Carbapenemase production. Results: The Predominant isolate was Pseudomonas aeruginosa36%.Among 24 MDR Pseudomonas isolates 29.1% and 37.5%were confirmed as MBL producer by phenotypic Modified Hodge test &amp;combined disk test respectively and 13 were positive for OXA genes&amp;all were negative for IMP &amp; VIM genes by molecular method. Conclusion: The study concludes that Ceftazidime resistance should be considered as an indicator for detection of resistant enzymes pattern in antimicrobial susceptibility tests</p>