Volume : 5, Issue : 9, September - 2016

STUDY OF GIBBERELLIC ACID PRODUCTION BY IMMOBILIZED CELLS OF FUSARIUM MONILIFORME.

Dr. Rakeshkumar R. Panchal

Abstract :

<p>&nbsp;Production of Gibberellic acid using immobilized cells of Fusarium moniliforme Sheldon, isolated from wilted sugarcane plants, by employing various entrapment agents at 2.0 gm % level viz. sodium alginate, K &ndash; karrageenan and agar agar was carried out. Among the various gel entrapment agents evaluated, sodium alginate was found to be superior which has given 230 &mu;g/ml GA3 which was 1.5 to 2.0 time higher production than other agents. The GA3 productivity increases on 5th day and 6th day but decreases on 7th day in all the entrapment agents. Sodium alginate was better as it resulted in comparatively firm and stable beads. When the effect of bead size on GA3 production using sodium alginate was investigated it was found that the maximum GA3 production was observed in the beads of 2.5 mm diameter which was 300 &mu;g/ml GA3. Also as the size of beads increases the GA3 production decreases. This may be due to reduction in the effective area for diffusion as the bead diameter increases. The GA3 production was not improved by immobilization. The level of GA3 productivity decreased in compared to the free cells which had given 315 &mu;g/ml GA3. This may be due to the methods of immobilization used, which may have affected the metabolic properties of the cells. When comparison was made with ssf technique, it is clear that the ssf is better method for GA3 production and should be preferred for reducing the cost of GA3 production.</p>