Volume : 5, Issue : 10, October - 2016
Influence of zona pellucida thickness of oocyte maturation and in vitro fertilization in the cow in Iraq
Saad Akram Hatif, Noor Al Mustafa Imad Kadhim
Abstract :
<p> The aim of study to investigation the role of zona pellucida thickness in the oocytes maturation and in vitro fertilization in cows . This study included the collection of 200 female reproductive system from the abattoir .The ovaries collected and washing with the normal saline . Oocytes recovered via , aspiration and slicing, followed the maturation in TCM199 in Co2 incubator . Semen collected , diluents , and capacitation in order to In Vitro Fertilization . Under inverted microscopical examination the results revealed , oocytes classified in three types , complete surrounded by cumulus cells ,partial surrounded ,and denuded oocytes, synchronizing with deferent appearance of zona pellucida thickness , thin ,normal , and thick .The best thickness of zona pellucida in the oocyte ranged (15um- 17um) for successfully maturation and in vitro fertilization .</p>
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Cite This Article:
Saad Akram Hatif, Noor Al Mustafa Imad Kadhim Influence of zona pellucida thickness of oocyte maturation and in vitro fertilization in the cow in Iraq Global Journal For Research Analysis,Volume-5, Issue-10, October‾2016