Volume : 6, Issue : 1, January - 2017

Evaluation of Gene Xpert Mycobacterium tuberculosis Assay for the Detection of Rifampicin Resistant Mycobacterium tuberculosis among Pulmonary Patients: A Hospital Based Study

Dr Basavaraj M S

Abstract :

<p>&nbsp;<span style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">Conventional diagnostic methods for </span><i style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">Mycobacterium tuberculosis </i><span style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">are slow and/or lack sensitivity. A number of new diagnostic approaches have ought incremental improvements to detection and drug susceptibility testing; however, the technical complexity of these assays and their dependence on dedicated laboratory infrastructure have limited their adoption, especially in low-resource, high-burden settings.</span><sup style="font-family: Andalus, serif; text-align: justify;"> </sup><span style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">It ranks as the second leading cause of death from an infectious disease worldwide. My aim was to evaluate the performance of GeneXpert system for the detection of </span><span style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">Mycobacterium tuberculosis </span><span style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">with its Rifampicin resistance. The concentration of </span><span style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">M. tuberculosis </span><span style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">bacteria in clinical sputum samples can vary from over 107 to less than 20 CFU/ml.</span><sup style="font-family: Andalus, serif; text-align: justify;"> </sup><span style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">Sputum samples were collected from 135 clinically suspected multidrug resistant pulmonary tuberculosis</span><span style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">&nbsp; </span><span style="font-family: Andalus, serif; font-size: 12pt; text-align: justify;">(MDR-TB) patients.</span></p> <p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify;text-justify:inter-ideograph;line-height:normal;mso-layout-grid-align:&#10;none;text-autospace:none"><span lang="EN-IN" style="font-size:12.0pt;font-family:&#10;&quot;Andalus&quot;,serif"><o:p></o:p></span></p>