Volume : 6, Issue : 3, March - 2017

COMPARISON OF ANTIOXIDANT EFFECT OF GLUTATHIONE AND ASTAXANTHINE IN THEIR THERAPEUTIC RANGE USING IN- VITRO METHODS

Dr. Sadhana C, Dr. Muthiah N. S

Abstract :

<p>&nbsp;<b><span lang="EN-IN" style="font-size:12.0pt;font-family:&quot;Arial&quot;,sans-serif;mso-fareast-font-family:&#10;&quot;Times New Roman&quot;;color:#724128;mso-fareast-language:EN-IN">Background and Aims:</span></b></p> <p class="MsoNormal" style="margin: 8.3pt 0in; line-height: normal; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"><span lang="EN-IN" style="font-size: 12pt; font-family: &quot;Times New Roman&quot;, serif;">Combined&nbsp; spinal&ndash;epidural (CSE) &nbsp;anaesthesia &nbsp;&nbsp;is&nbsp; &nbsp;being&nbsp; &nbsp;increasingly &nbsp;&nbsp;used&nbsp; for&nbsp; effective post-operative &nbsp;analgesia. This &nbsp;study &nbsp;was &nbsp;designed &nbsp;to &nbsp;evaluate &nbsp;the &nbsp;effect &nbsp;of&nbsp; epidural dexmedetomidine &nbsp;on &nbsp;characteristics &nbsp;of &nbsp;spinal &nbsp;anaesthesia &nbsp;for &nbsp;lower &nbsp;abdominal &nbsp;surgeries.<o:p></o:p></span></p> <p class="MsoNormal" style="margin: 15.4pt 0in 7.7pt; line-height: 15pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"><b><span lang="EN-IN" style="font-size:12.0pt;font-family:&quot;Arial&quot;,sans-serif;mso-fareast-font-family:&#10;&quot;Times New Roman&quot;;color:#724128;mso-fareast-language:EN-IN">Methods:<o:p></o:p></span></b></p> <p class="MsoNormal" style="margin: 8.3pt 0in; line-height: normal; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"><span lang="EN-IN" style="font-size: 12pt; font-family: &quot;Times New Roman&quot;, serif;">This &nbsp;was &nbsp;a &nbsp;prospective &nbsp;randomised, &nbsp;double-blind, &nbsp;controlled &nbsp;study &nbsp;involving &nbsp;fifty patients &nbsp;belonging &nbsp;to &nbsp;American &nbsp;Society &nbsp;of &nbsp;Anesthesiologists(ASA) &nbsp;&nbsp;&nbsp;Physical Status I &nbsp;and &nbsp;II who &nbsp;underwent &nbsp;surgery were randomly divided into dexmedetomidine( D) group and saline (S) group of twenty five each. All patients received CSE anaesthesia. Ten minutes before subarachnoid block (SAB), Group D received dexmedetomidine1 &mu;g/kg diluted to 5 ml in normal saline (NS) and Group S received NS epidurally. Hyperbaric bupivacaine (15 mg) was administered intrathecally for both groups after epidural injection. Sensory and motor block characteristics, analgesia, sedation and haemodynamics were observed. Statistical analysis was performed using appropriate tests.<o:p></o:p></span></p> <p class="MsoNormal" style="margin: 15.4pt 0in 7.7pt; line-height: 15pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"><b><span lang="EN-IN" style="font-size:12.0pt;font-family:&quot;Arial&quot;,sans-serif;mso-fareast-font-family:&#10;&quot;Times New Roman&quot;;color:#724128;mso-fareast-language:EN-IN">Results:<o:p></o:p></span></b></p> <p class="MsoNormal" style="margin: 8.3pt 0in; line-height: normal; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"><span lang="EN-IN" style="font-size: 12pt; font-family: &quot;Times New Roman&quot;, serif;">Epidural &nbsp;dexmedetomidine &nbsp;as adjuvant to SAB in CSE &nbsp;produced faster onset and prolonged duration of sensory and motor blockade. There was prolonged duration of analgesia with improved sedation and low pain scores in post operative period in the dexmedetomidine group.<o:p></o:p></span></p> <p class="MsoNormal" style="margin: 15.4pt 0in 7.7pt; line-height: 15pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"><b><span lang="EN-IN" style="font-size:12.0pt;font-family:&quot;Arial&quot;,sans-serif;mso-fareast-font-family:&#10;&quot;Times New Roman&quot;;color:#724128;mso-fareast-language:EN-IN">Conclusion:<o:p></o:p></span></b></p> <p class="MsoNormal" style="margin: 8.3pt 0in; line-height: normal; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"><span lang="EN-IN" style="font-size: 12pt; font-family: &quot;Times New Roman&quot;, serif;">Administration&nbsp; of&nbsp; dexmedetomidine&nbsp; epidurally, 10 min before SAB, caused early onset and prolonged duration of motor blockade and analgesia, without any significant post-operative complication.<o:p></o:p></span></p>