Volume : 6, Issue : 5, May - 2017

Screening of Keratinolytic Bacteria and Keratinase Production by using Pseudomonas aeruginosa isolated from Poultry

Patil R. C. , Jadhav B. L.

Abstract :

<p>&nbsp;Enzymes keratinase used for degradation of keratins and it is found in animal hair, nails, hoofs and feathers. Feather waste is generated in large amounts as a by-product of commercial poultry processing and cause serious environmental problems. In the present study a total of 25 strains were isolated and identified based on morphological and biochemical Characterization and analysis of 16S rDNA sequences. Among them a potential feather degrading organism Pseudomonas aeruginosa K13 was shown highest Keratinolytic activity and its protein concentration was 280 U/ml. The intense feather degradation was achieved at 400C and at pH to 7 and 8. The metal ions including Zn2+ and Cd2+ enhance the keratinolytic activity and Hg2+ and Mg2+ inhibits the keratinolytic activity. The activity of 60% precipitate enzyme was found to be 1056 U/ml. This precipitate was subjected to Ion exchange chromatography for further purification process. The fraction number 17 shows 11.74 mg/ml concentration of protein and enzymatic activity was found to be 11.44 U/ml.&nbsp; From the gel, it was observed that molecular mass of keratinase of K13 isolate was 38 kDa. The potential bacterial isolates can be used for degradation of feathers waste of commercial poultry processing. &nbsp;</p>