Volume : 6, Issue : 6, June - 2017

Study of association of Leptin, Sex hormone binding globulin (SHBG) and Glucose-insulin ratio (GIR) with 25-Hydroxycholecalciferol for insulin resistance in women with Poly Cystic Ovarian Syndrome

Dr Huma Nasrat, Akanksha Dubey

Abstract :

Introduction-PCOS has emerged as a major endocrinological disorder in females in the reproductive age group with significant short term and long term effects such as metabolic syndrome infertility, cardiovascular disorders. Insulin resistance is one of the cardinal features of this syndrome. Aims and objectives-To study the different biochemical and anthropometric markers of insulin resistance and to correlate with vitamin D status in PCOS patients and to explore whether vitamin D could be considered as predictor of insulin resistance in PCOS.<o:p></o:p> Materials and methods-Sixty patients of PCOS diagnosed by Rotterdam criteria were enrolled in this study.Estimation of 25- OH vitamin D was done by ELISA using 25 OH vitamin D direct ELISA kit from Immundiagnostik (Bensheim). Serum leptin level was measured by ELISA by using leptin DRG leptin sandwich ELISA kit. Sex hormone binding globulin was measured by ELISA using DRG SHBG. Insulin levels were estimated by Chemiluminescence immunoassay (CLIA) in Access 2 Beckmann Coulter.<o:p></o:p> Results-28.34% of PCOS women showed 25(OH)D values <30 nmol/L(Vitamin D deficiency).25-OH-vit D levels were significantly different in the two groups (HOMA-IR≤ 3.9 and HOMA>3.9).We found a significant negative correlation  of 25(OH)D levels with fasting  insulin, HOMA-IR and glucose –insulin ratio.We found that vitamin D was the important determinant of HOMA-IR in  women with PCOS.28.9 % of total variation in HOMA-IR is explained by 25-OH-vit D alone (r2=0.289 p value 0.0001).<o:p></o:p> <span lang="EN-IN" style="font-size:12.0pt;
line-height:115%;font-family:"Times New Roman",serif;mso-fareast-font-family:
Calii;mso-fareast-theme-font:minor-latin;mso-bidi-font-family:Mangal;
mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-IN;mso-fareast-language:
EN-US;mso-bidi-language:AR-SA">Conclusions<span lang="EN-IN" style="font-size:12.0pt;line-height:115%;font-family:"Times New Roman",serif;
mso-fareast-font-family:Calii;mso-fareast-theme-font:minor-latin;mso-bidi-font-family:
Mangal;mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-IN;mso-fareast-language:
EN-US;mso-bidi-language:AR-SA">-<span lang="EN-IN" style="font-size:12.0pt;
line-height:115%;font-family:"Times New Roman",serif;mso-fareast-font-family:
Calii;mso-fareast-theme-font:minor-latin;mso-ansi-language:EN-IN;mso-fareast-language:
EN-US;mso-bidi-language:AR-SA">Lower levels of vitamin D are associated with higher levels of insulin resistance and thus Vitamin D deficiency might be involved in the pathogenesis of insulin resistance in PCOS.

Keywords :

Article:

Download PDF Journal DOI : 10.15373/2249555X

Cite This Article:

Dr Huma Nasrat, Akanksha Dubey, Study of association of Leptin, Sex hormone binding globulin (SHBG) and Glucose-insulin ratio (GIR) with 25-Hydroxycholecalciferol for insulin resistance in women with Poly Cystic Ovarian Syndrome, GLOBAL JOURNAL FOR RESEARCH ANALYSIS : VOLUME-6 | ISSUE‾6 | JUNE-2017