Volume : 6, Issue : 7, July - 2017

M. tuberculosis in Bone Marrow as commensal � A hidden source for primary osteoarticular Tuberculosis !!

Vidyut Prakash, Deepak Kumar, Saurabh Singh, Gopal Nath

Abstract :

<p>&nbsp;<b style="text-align: justify;"><span lang="EN-IN" style="font-size:12.0pt;line-height:115%;font-family:&#10;&quot;Times New Roman&quot;,serif">BACKGROUND:</span></b></p> <p class="MsoNormal" style="text-align:justify"><span lang="EN-IN" style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,serif">The bone marrow of healthy individuals is conventionally considered sterile like other body fluids, but recently the scientists found genetic material from the bacteria inside their stem cells. The findings raise the possibility that other infectious agents may also reside in the bone marrow. </span><span style="font-size:12.0pt;line-height:115%;&#10;font-family:&quot;Times New Roman&quot;,serif;mso-ansi-language:EN-US"><o:p></o:p></span></p> <p class="MsoNormal" style="text-align:justify"><b><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-ansi-language:EN-US">OBJECTIVES:<o:p></o:p></span></b></p> <p class="MsoNormal" style="text-align:justify"><span style="font-size:12.0pt;&#10;line-height:115%;font-family:&quot;Times New Roman&quot;,serif;mso-ansi-language:EN-US">The study was planned to look for the presence of all type of bacteria by amplifying 16S rDNA sequences by using eubacterial universal primers. <o:p></o:p></span></p> <p class="MsoNormal" style="text-align:justify"><b><span lang="EN-IN" style="font-size: 12pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">MATERIAL AND METHODS:<o:p></o:p></span></b></p> <p class="MsoNormal" style="text-align:justify"><span lang="EN-IN" style="font-size: 12pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">A total of 18 bone marrow samples of apparently healthy individuals were collected from patients admitted </span><span lang="EN-IN" style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,serif">having closed bone fractures. The DNA was isolated and was subjected to nested PCR using Universal eubacterial 16S rDNA primers. The samples positive by universal PCR was further checked for the presence of <i>S</i>. Typhi, <i>S</i>. ParaTyphi, A and or<i> M. tuberculosis</i> if any using a second nested PCR reaction. <o:p></o:p></span></p> <p class="MsoNormal" style="text-align:justify"><b><span style="font-size: 12pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">RESULTS:<o:p></o:p></span></b></p> <p class="MsoNormal" style="text-align:justify"><span style="font-size: 12pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">A total of 16 (89%) samples could yield the desired amplicon through universal PCR. The secondary PCR of 16 samples, the</span><span lang="EN-IN" style="font-size: 12pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;"> desired amplicons were detected 3 (18%) for </span><i><span lang="EN-IN" style="font-size:12.0pt;&#10;line-height:115%;font-family:&quot;Times New Roman&quot;,serif">M. Tuberculosis,</span></i><span lang="EN-IN" style="font-size: 12pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;"> 4 (22%) for S.Typhi, and 1 (6%) for ParaTyphi A. <o:p></o:p></span></p> <p class="MsoNormal" style="text-align:justify"><b><span lang="EN-IN" style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-bidi-font-style:italic">CONCLUSIONS:<o:p></o:p></span></b></p> <p class="MsoNormal" style="text-align:justify"><span lang="EN-IN" style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-bidi-font-weight:bold;mso-bidi-font-style:italic">Even in asymptomatic cases other infectious agents like </span><i><span lang="EN-IN" style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-bidi-font-weight:bold">M. tuberculosis,</span></i><span lang="EN-IN" style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-bidi-font-weight:bold;mso-bidi-font-style:italic"> </span><i><span lang="EN-IN" style="font-size:12.0pt;&#10;line-height:115%;font-family:&quot;Times New Roman&quot;,serif;mso-bidi-font-weight:bold">Salmonella spp</span></i><span lang="EN-IN" style="font-size:12.0pt;line-height:115%;&#10;font-family:&quot;Times New Roman&quot;,serif;mso-bidi-font-weight:bold;mso-bidi-font-style:&#10;italic">. and many other microorganisms may be present as commensal and may cause active clinical infection on certain conditions.<o:p></o:p></span></p>