Volume : 6, Issue : 7, July - 2017

Microbial Diversity analysis of Dal lake, India using 16S rRNA Gene Based Culturing Approach.

Khan J. A

Abstract :

<p>&nbsp;<span style="text-align: justify; font-size: 12pt;">Dal lake ecosystem (Lat. 340-6&rsquo; N, 740-45&rsquo;E, alt.1583) situated in the heart of Srinagar city the summer capital of Jammu and Kashmir state is under tremendous anthropogenic pressure for the last there decades.More than 50,000 people are living inside this hamlet, besides some people residing inside houseboats. People use this lake &nbsp;for their own purposes as this lake acts as livelihood for many families since centuries. Little work had been carried on Dal lake water and sediment samples to show the type of &nbsp;Microbial Diversity existing at present</span><span style="text-align: justify; font-size: 11.5pt; font-family: &quot;Times New Roman&quot;, serif;">. By our work we tried to show whether anthropogenic activity is responsible for producing harmful bacteria. The traditional method of Microbial diversity analysis, culture dependent identification of species through morphological and biochemical test has not given the oad range of organism present in Dal Lake.&nbsp; Till date, only <i>Escherichia coli</i>, <i>Salmonella typhimurium </i>and <i>Penicillium </i>sp. were only identified. We have utilized 16S rRNA approach &nbsp;first time to study Microbial diversity in detail by taking water and sediment samples and had grown them on different media like Nutrient agar, Blood agar, and Eosine Methyline blue agar. &nbsp;As studies of 16S rRNA provides a microbial taxa present in a given sample because it is an excellent phylogenetic marker. Bacterial 16S rRNA sequences show the distribution of microorganisms predominantly in <i>Bacillus, Acinetobacter </i>and <i>Pseudomonas </i>species. As by our findings sediment samples show a variety of bacteria, whereas water samples showed presence of very few types of bacteria. The other bacteria recovered from the sediment samples include bacteria belonging to <i>Sporosarcina</i>, <i>Streptomyces</i>, <i>Arthrobacter</i>, <i>Rhodococcus</i>, <i>Enterobacter</i>, <i>Hydrogenophaga</i>, <i>Rheinheimera</i>, and <i>Gammaproteobacterium </i>species. The other isolate recovered from the water samples belong to the species <i>Comamonas</i>, <i>Micrococcus</i>, <i>Rheinheimera</i>, <i>Acidovorax</i>, <i>Sporosarcina</i>, <i>Paenisporosarcina</i>, <i>Rhodococcus</i>, <i>Bosea</i>, <i>Arthrobacter</i>. Based on literature survey, we can utilise the services of these bacteria for various &nbsp;purposes as these&nbsp; microorganisms can ing bioremediation activity in Dal lake such as degradation of crude oil, aromatic and alicyclic compounds, polynuclear aromatic hydrocarbons, mineral oils, synthetic polymers, styrene, heavy metals.&nbsp;</span></p> <p class="MsoNormal" style="text-align:justify"><span style="font-size: 11.5pt; font-family: &quot;Times New Roman&quot;, serif;"><o:p></o:p></span></p>