Volume : 3, Issue : 5, May - 2014

Improved L–Methionine Fermentation With Immobilized Corynebacterium Glutamicum X300

Subhadeep Ganguly, Kunja Bihari Satapathy

Abstract :

An experimental study was conducted to examine the advantage of whole cell immobilization of a mutant Corynebacterium glutamicum X300 entrapped into calcium alginate beads for L–methionine production. For this purpose, several parameters were examined to maximize the product yield. Production was increased significantly (p<0.01) with immobilized Corynebacterium glutamicum X300 into calcium alginate compared to the production by free cells. Production was comparatively lower with the immobilized cells into chitosan–coated calcium alginate beads. Maximum production of L–methionine was obtained with 2.5% sodium alginate, 0.3 (M) CaCl2, 6.0 mm bead diameter and 24h storage period,using 0.4 (M) CaCl2 in the synthetic medium for bead stability. A continuous production of L–methionine was obtained up to 66th cycle reuse of the beads.