Volume : 4, Issue : 1, January - 2015

Studies on Designing, Optimisation and Application of Biosensor using Glucose Oxidase of Aspergillus niger F-C405-2

Ms. Gothoskar Vandan, Dr. Mrs. Smita M. Dharmadhikari, Dr. Mahendra D. Shirsat

Abstract :

<p>Biosensor is a device which detects and combines with a biological component analysed with a physicochemical< /> detector component. In present work we have designed a glucose biosensor using immobilised glucose oxidase enzyme< /> on cellulose acetate produced by Aspergillus niger F&ndash; C405&ndash;2. This glucose oxidase (GO) has shown thermal stability at< /> 600C for 120 minutes. Glucose oxidase was immobilized on pyrrol&ndash;cellulose acetate (Py&ndash;CA) film over Indium tin oxide (ITO). Platinum and silversilver< /> chlorides are used as reference electrode and suitable for estimating blood sugar with commercial glucometer. The biosensor prepared< /> was optimised and caliated against standard glucose solutions of variable concentration (0.5 to 6 mg/ml) with change in electric current< /> measured in ampere. The amount of glucose present in patient&rsquo;s blood was estimated using our designed biosensor and diagnosed for diabetes.< /> Our instrumental results were found to be correlated with that measured using commercial glucometer. No significant deviation was observed. </p>