Volume : 4, Issue : 2, February - 2015

SCREENING AND CHARACTERIZED THE ANTAGONISTIC FUNGUS OF Trichoderma koningii AGAINST Colletotrichum falcatum BY FOOD POISONING TECHNIQUE

Prince. L

Abstract :

<p>Biocontrol is environmentally safe and in some cases is the only option available to protect plants against pathogens (Cook, 1993). The present study were screened the presence of antagonistic potentials of selected fungal isolates against Colletotrichum falcatum by food poisoning technique and characterized the antagonistic fungus of Trichoderma koningii. Colletotrichum falcatum Went causing Red rot disease in sugarcane was isolated from the infected sett in sugarcane field in Tamilnadu, India. The antagonistic fungus were screening of by dual culture experiments and food poisoning method. The maximum percentage inhibition of growth of C. falcatum was found with Trichoderma koningii (75%) followed by Botrytis cinerea (73.8%), T. glaucum (72.3%), T. viride (70%), Penicillium chrysogenum (69.2%), Gliocladium virens (56.9%), T. harzianum (53.8%) and P. citrinum (23.1%). The potential biocontrol of Trichoderma koningii was molecular characterized. The phylogenetic analysis revealed that 598 bps sequence of ITS region of Hypocrea koningii PPTK was similar (99%) to the existing species of Hypocrea koningii CY059 (GenBank Accession Number HQ607942).</p>