Volume : 1, Issue : 7, December - 2012

Antibiotics Susceptibility Patterns of Bacterial Isolates among Ventilator Associated Pneumonia in Tertiary Care Hospital, Jamnagar, Gujarat

Dr. Swati Pathak, Dr. Hitesh Shingala, Dr. Hiral Shah, Dr. Beena Jagad, Dr. Viral Shah, Dr. Mala Sinha

Abstract :

Introduction :Ventilator–associated pneumonia (VAP) is defined as pneumonia occurring more than 48 hrs after patients have been intubated and received mechanical ventilation. Diagnosing VAP requires a high clinical suspicion combined with bedside examination, radiographic examination and microbiological analysis of respiratory secretions Material and method: Samples were either aspirated by disposable syringes and transferred into sterile glass test tubes or collected onto sterile cotton wool swabs, aseptically and inoculated on Blood agar and Mac Conkey’s agar. After 24 hours of incubation at 37? c of all agar plates, culture Characteristics of colony were observed. Gram stain of colony were done and according to gram positive or gram negative bacteria they were proceed for biochemical tests and antibiotic sensitivity testing. Results: Out of total 250 samples, 135 (54) shows growth. From 135 positive cultures, predominant organisms were Pseudomonas aeruginosa 60 (41.37%) followed by S. aureus 34 (23.44%), Maximum sensitive drug for Gram negative bacteria was Colistin , for gram positive bacteria was Vancomycin and for pseudomonas was Meropenem