Volume : 5, Issue : 4, April - 2016

Effect of cyclophosphamide on the microanatomy of lungs of albino rats

Javed Ahmad Khan, Yasmeen Jan, Sajad Hamid, Sabia Nazir, Zahid Bashir, Shameema Banoo, Mohd. Arif

Abstract :

<p>&lt;p&gt;&amp;lt;p&amp;gt;&amp;amp;nbsp;Background: Cyclophosphamide is one of the many drugs used for the treatment of many neoplastic diseases and diseases associated with altered immunity. Organs like heart, reproductive organs, urinary bladder and lungs are effected when higher doses of cyclophosphamide are used for longer durations.Aim:our aim is thus to see the effects of cyclophosphamide on lungs on chronic exposure Methods: Sixty eight Albino rats were taken to study the effects of cyclophosphamide on the microanatomy of lungs. The animals were divided into three groups, group A ( control group) of 20 animals, were fed with routine diet, group B ( low dose group) of 24 animals, were given cyclophosphamide at the dose of 0.5 mg/100gms in addition to the routine diet and group C ( high dose group) of 24 animals were given high dose of cyclophosphamide at the dose of 0.7gm/100gms of weight of animals in addition to the routine diet. The animals were sacrificed at intervals of 3, 6, 9 and 12 weeks, 5 microns sections of the tissues were prepared and stained with Haematoxylin and Eosin stain and the stained slides are observed under light microscopy.Results: Microscopic changes were seen in the lungs of drug treated animals. The microscopic changes started appearing after third week of drug administration. The changes were in the form of damage to endothelium of pulmonary vasculature with areas of fresh haemorrhage, damage to alveolar epithelium and interstitial fiosis. Conclusion: Cyclophosphamide induces histological changes in the form of damage to endothelium of pulmonary vasculature, damage to alveolar epithelium and interstitial fiosis. Thus patients receiving cyclophosphamide need to be continuously monitored for pulmonary dysfunction.&amp;lt;/p&amp;gt;&lt;/p&gt;</p>