Volume : 6, Issue : 6, June - 2017

SIMULTANEOUS EVALUATION, ESTIMATION OF HEPATOPROTECTIVE EFFECTS OF KYLLINGA BREVIFOLIA AND ANTI-EPILEPTIC ACTIVITY OF RORIPPA SARMENTOSA EXTRACTS IN RATS

Dr. Syed Arshaduddin Ahmed, Dr. K. Chakravarthy

Abstract :

<p>&nbsp;Methanolic extract of whole plant of Kyllinga evifolia (MEKB) was prepared and investigation of its hepatoprotective activity and acute oral toxicity was carried out in male Wistar albino rats by using CCl4 induced hepatotoxicity. 150 and 300mg/kg, p.o doses of MEKB and 100mg/kg, p.o dose of the standard drug Silymarin were administered three times at intervals of 8hr and then 0.8ml/kg of CCl4 was administered to all the groups except normal control for 2 days. Observation of various biochemical parameters like SGOT, SGPT, ALP, &gamma;-GT, TP and total bilirubin to assess the hepatoprotective activity along with histopathological studies was carried out after 24h of CCl4 treatment. As assessed by the biochemical changes and histopathological studies, MEKB at the doses of 150 and 300mg/kg inhibited CCl4 induced liver toxicity in Wistar albino rats. The methanolic extract of whole plant of Kyllinga evifolia demonstrated significant protection against CCl4 inducd hepatocellular injury. In traditional Indian medicine, the whole plant of Rorippa sarmentosa (DC.) Mac. is used to treat epilepsy. Previous studies have demonstrated that extracts of these plants was subjected to acute toxicity and then screened for antiepileptic activity on Maximal Electroshock (MES) and Pentylenetetrazole (PTZ) induced seizures models in albino wistar rats. The aim of the present study is the investigation of the effect of ethanolic (95%) extract of Rorippa sarmentosa (DC.) Mac. (EERS) on biogenic amines concentrations in rat ain after induction of seizures by MES and PTZ. &nbsp;The relationship between seizure activities and altered concentrations of monoamines such as noradrenaline (NA), dopamine (DA), serotonin (5-HT) and Gamma amino butyric acid (GABA) in foreain of rats in MES and PTZ seizure models was our aim of study. In MES model, EERS (150 &amp; 300 mg/kg) significantly restored the decreased levels of ain monoamines such as NA, DA, 5-HT and GABA. Similarly, EERS significantly increased the monoamines in foreain of rats in PTZ model. Hence, we can conclude that there was an increase in the monoamines concentration in rat ain upon administration of the ethanolic extract of Rorippa sarmentosa (DC.) Mac., which may decrease the susceptibility to MES and PTZ induced seizure in rats.</p>