Volume : 6, Issue : 11, November - 2017

Phenotypic detection of carbapenemase production and molecular detection of blaOXA-51 and blaNDM-1 genes among the carbapenem resistant Enterobacteriaceae in a tertiary care rural teaching hospital

Dr. M. Jane Esther, Dr. Diego Edwin

Abstract :

<p>&nbsp;<b style="text-align: justify;"><span lang="EN-IN">Introduction:</span></b></p> <p class="MsoNormal" style="text-align:justify"><span lang="EN-IN">CRE contribute to increased morbidity and mortality among hospitalized patients. A knowledge of CRE prevalence helps in treatment and prevention of further spread of resistance.<o:p></o:p></span></p> <p class="MsoNormal" style="text-align:justify"><b><span lang="EN-IN">Materials and methods:<o:p></o:p></span></b></p> <p class="MsoNormal" style="text-align:justify"><span lang="EN-IN">All meropenem resistant Enterobacteriaceae isolates were subjected growth on KPC chrome agar, MHT, EDS test, CDT and genotyping for detection of baOXA-51 and blaNDM-1 genes.<o:p></o:p></span></p> <p class="MsoNormal" style="text-align:justify"><b><span lang="EN-IN">Results and discussion:<o:p></o:p></span></b></p> <p class="MsoNormal" style="text-align:justify"><span lang="EN-IN">Prevalence of CRE was 3.5%(25/713). 24/25(96%) CRE grew on KPC chrome agar, 64%(16/25) were positive for MHT, 80%(20/25) each for EDS and CDT. 13/25 harboured blaNDM-1, one blaOXA-51 and 7 both. We found good consistency between phenotypic and genotypic results. Ours is the pioneer study in India in detecting blaOXA-51 among Enterobacteriaceae. <o:p></o:p></span></p> <p class="MsoNormal" style="text-align:justify"><b><span lang="EN-IN">Conclusion:<o:p></o:p></span></b></p> <p class="MsoNormal" style="text-align:justify"><span lang="EN-IN">Even though the prevalence of CRE is low, appropriate infection control measures are needed to prevent further spread of resistance.&nbsp;&nbsp;<o:p></o:p></span></p>