Volume : 7, Issue : 2, February - 2018

A comparative study of Ziehl Neelsen, Gabbet‘s and Fluorescent staining methods to detect acid fast bacilli in sputum samples.

Ms. Sharon Priya P. J, Dr. Thomas. S. Kuruvilla

Abstract :

<p>&nbsp;</p> <p class="MsoNormal" style="line-height:150%"><span style="font-size:12.0pt;&#10;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-bidi-font-weight:&#10;bold"><span style="mso-spacerun:yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>Tuberculosis <span style="mso-spacerun:yes">&nbsp;</span>remains <span style="mso-spacerun:yes">&nbsp;</span>world&rsquo;s <span style="mso-spacerun:yes">&nbsp;</span>leading <span style="mso-spacerun:yes">&nbsp;</span>cause <span style="mso-spacerun:yes">&nbsp;</span>of <span style="mso-spacerun:yes">&nbsp;</span>death <span style="mso-spacerun:yes">&nbsp;</span>from <span style="mso-spacerun:yes">&nbsp;</span>a <span style="mso-spacerun:yes">&nbsp;</span>single <span style="mso-spacerun:yes">&nbsp;</span>infectious agent. Pulmonary <span style="mso-spacerun:yes">&nbsp;</span>tuberculosis (PTB) is <span style="mso-spacerun:yes">&nbsp;</span>the <span style="mso-spacerun:yes">&nbsp;</span>most <span style="mso-spacerun:yes">&nbsp;</span>common <span style="mso-spacerun:yes">&nbsp;</span>presentation <span style="mso-spacerun:yes">&nbsp;</span>of <span style="mso-spacerun:yes">&nbsp;</span>tuberculosis. </span><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Here we compare Ziehl-Neelsen (ZN),</span><i><span style="font-size:9.0pt;&#10;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"> </span></i><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-bidi-font-style:italic">Gabbet&rsquo;s <span style="mso-spacerun:yes">&nbsp;</span>and <span style="mso-spacerun:yes">&nbsp;</span>Fluorescent staining (FS) methods <span style="mso-spacerun:yes">&nbsp;</span>to <span style="mso-spacerun:yes">&nbsp;</span>determine <span style="mso-spacerun:yes">&nbsp;</span>efficiency <span style="mso-spacerun:yes">&nbsp;</span>and cost effectiveness of diagnosing<span style="mso-spacerun:yes">&nbsp; </span>pulmonary tuberculosis<span style="mso-spacerun:yes">&nbsp;&nbsp; </span>from sputum<span style="mso-spacerun:yes">&nbsp; </span>samples.</span><span style="font-size:12.0pt;line-height:150%;&#10;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"> <span style="mso-bidi-font-style:italic">Three <span style="mso-spacerun:yes">&nbsp;</span>smear<span style="mso-spacerun:yes">&nbsp; </span>from 148 sputum <span style="mso-spacerun:yes">&nbsp;</span>samples<span style="mso-spacerun:yes">&nbsp; </span>of<span style="mso-spacerun:yes">&nbsp; </span>PTB cases</span></span><span style="font-size:12.0pt;line-height:150%;font-family:&#10;&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:Calii"> were prepared and stained by the above methods.</span><b style="mso-bidi-font-weight:normal"><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-bidi-font-style:italic"> </span></b><span style="font-size:12.0pt;&#10;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-bidi-font-style:&#10;italic">Of <span style="mso-spacerun:yes">&nbsp;</span>the <span style="mso-spacerun:yes">&nbsp;</span>samples <span style="mso-spacerun:yes">&nbsp;</span>examined, 71.6% and 53.4% and 85.8% cases were detected <span style="mso-spacerun:yes">&nbsp;</span>by <span style="mso-spacerun:yes">&nbsp;</span></span><span style="font-size:12.0pt;&#10;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Ziehl-Neelsen,</span><i><span style="font-size:9.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"> <span style="mso-spacerun:yes">&nbsp;</span></span></i><span style="font-size:12.0pt;&#10;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-bidi-font-style:&#10;italic">Gabbet&rsquo;s<span style="mso-spacerun:yes">&nbsp; </span>and<span style="mso-spacerun:yes">&nbsp; </span>Fluorescence <span style="mso-spacerun:yes">&nbsp;</span>staining <span style="mso-spacerun:yes">&nbsp;</span>respectively</span><b style="mso-bidi-font-weight:&#10;normal"><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">. </span></b><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-bidi-font-style:italic">Fluorescent staining <span style="mso-spacerun:yes">&nbsp;</span>was <span style="mso-spacerun:yes">&nbsp;</span>found to be more reliable and cost effective method <span style="mso-spacerun:yes">&nbsp;</span>particularly<span style="mso-spacerun:yes">&nbsp; </span>when <span style="mso-spacerun:yes">&nbsp;</span>dealing with <span style="mso-spacerun:yes">&nbsp;</span>large sample loads than </span><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Ziehl-Neelsen<span style="mso-spacerun:yes">&nbsp; </span>and<span style="mso-spacerun:yes">&nbsp; </span><span style="mso-bidi-font-style:italic">Gabbet&rsquo;s <span style="mso-spacerun:yes">&nbsp;</span>staining technique.<o:p></o:p></span></span></p>