Volume : 7, Issue : 5, May - 2018

SYNTHESIS, CHARACTERIZATION AND APPLICATION OF NANOPARTICLES IN A NOVEL SKIN FORMULATION

Dugal Suparna, Shaikh Ayesha

Abstract :

<p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify;line-height:115%"><span lang="EN-IN" style="font-size:12.0pt;line-height:&#10;115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;&#10;mso-fareast-theme-font:minor-fareast;color:#0D0D0D;mso-themecolor:text1;&#10;mso-themetint:242">Human skin is prone to many challenges like infections, climate change and environmental stress. The current study employs a novel approach in formulating a skin preparation possessing both antibacterial and anti-oxidant activity.<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify;line-height:115%"><span lang="EN-IN" style="font-size:12.0pt;line-height:&#10;115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;&#10;mso-fareast-theme-font:minor-fareast;color:#0D0D0D;mso-themecolor:text1;&#10;mso-themetint:242">In the present study silver nanoparticles and zinc oxide nanoparticles were chemically synthesized and characterized by UV- visible spectroscopy and the maxima absorption was obtained at 430nm and 370nm respectively.<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify;line-height:115%"><span lang="EN-IN" style="font-size:12.0pt;line-height:&#10;115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;&#10;mso-fareast-theme-font:minor-fareast;color:#0D0D0D;mso-themecolor:text1;&#10;mso-themetint:242"><span style="mso-spacerun:yes">&nbsp;</span>Antimicrobial activity of the synthesized nanoparticles was tested by the agar cup diffusion assay against <i style="mso-bidi-font-style:normal">E.coli</i> and <i style="mso-bidi-font-style:normal">S.aureus</i> as the test organisms. MIC of the synthesized silver nanoparticles was found to be 45.85 ppm whereas, for zinc oxide nanoparticles it was found to be 109.5ppm. Atomic absorption spectroscopy studies revealed the concentration of silver nanoparticles and zinc oxide nanoparticles to be 50.4ppm and 1752 ppm respectively. Scanning electron microscopy revealed that silver nanoparticles were spherical in morphology and approximately 67 nm in diameter, while zinc oxide nanoparticles were approximately 75nm in diameter with rod shape morphology.<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify;line-height:115%"><span lang="EN-IN" style="font-size:12.0pt;line-height:&#10;115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;&#10;mso-fareast-theme-font:minor-fareast;color:#0D0D0D;mso-themecolor:text1;&#10;mso-themetint:242">An antibacterial lotion was prepared with the above-mentioned nanoparticles incorporated in it. Further, in order to impart antioxidant activity of the lotion, ascorbic acid was incorporated. Assays performed revealed the lotion to possess good antimicrobial and antioxidant activity.<o:p></o:p></span></p>