Volume : 7, Issue : 6, June - 2018

ANTI BACTERIAL EFFECT OF SILVER NANOPARTICLE ON MRSA ISOLATES FROM ORAL INFECTIONS

Dr. Zameer Pasha, Dr. Savina Gupta, Dr. Nazia Khan

Abstract :

<p class="MsoNormal" style="tab-stops:.5in right 6.0in"><span style="font-size:&#10;12.0pt;line-height:107%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-ascii-theme-font:&#10;major-bidi;mso-hansi-theme-font:major-bidi;mso-bidi-theme-font:major-bidi">Aim of our study is to evaluate the antibacterial activity of silver Nano particle(Ag-NPs) against MRSA strains recovered from patients with oral infections<o:p></o:p></span></p> <p class="MsoNormal" style="text-align:justify;text-indent:.5in;line-height:115%"><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-ascii-theme-font:major-bidi;mso-hansi-theme-font:major-bidi;mso-bidi-theme-font:&#10;major-bidi">Material and Methods:-<span style="mso-spacerun:yes">&nbsp; </span>Samples were collected from Patients attending department of oral and maxillofacial surgery, Jaipur dental college who were <span style="mso-bidi-font-weight:bold">diagnosed with oral infections. The samples were subjected to culture and sensitivity and MRSA (Methicillin resistant </span><span style="background:white">Staphylococcus aureus</span><span style="mso-bidi-font-weight:bold">) screening. MRSA</span>strains were treated with Ag-Nanoparticles to know the antibacterial affect <o:p></o:p></span></p> <p class="MsoNormal" style="text-align:justify;line-height:115%"><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-ascii-theme-font:major-bidi;mso-hansi-theme-font:major-bidi;mso-bidi-theme-font:&#10;major-bidi">Results:- Out of 146 samples, 26 specimens showed Methicillin-resistant S aureus (MRSA) The MIC and MBC values of Ag-NPs against MRSA strains were observed to be very low (i.e. in the range of 12.5-100 &mu;g/ml), indicating very well bacteriostatic (represented by the MIC) and bactericidal activity (represented by MBC).<span style="mso-spacerun:yes">&nbsp; </span><o:p></o:p></span></p> <p class="MsoNormal" style="text-align:justify;line-height:115%"><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-ascii-theme-font:major-bidi;mso-hansi-theme-font:major-bidi;mso-bidi-theme-font:&#10;major-bidi">Conclusion:- In recent years, there is increase in MRSA in oral and perioral samples with emergence of new resistant strains of MRSA. In our study we have seen Ag-NPs size 5-10nm has inhibited growth of Methicillin Resistant Staphylococcus aureus in vitro at nontoxic concentration, which makes it to use as anti-bacterial agent in oral infections<o:p></o:p></span></p>