Volume : 7, Issue : 5, May - 2018

Phenotypic characterization and biofilm detection of Coagulase negative Staphylococci isolated from clinical specimens in Kota region

Dr. Anita E. Chand, Dr. Danish, Dr. Pradhuman Singh Chauhan

Abstract :

Introduction: Coagulase negative staphylococci are most often associated with chronic infections of implemented medical devices. The biofilm-forming ability of CONS helps to resist the conditions of the surrounding environment with high levels of tolerance to antibodies, antibiotics, disinfectants and phagocytic inflammatory cells. So CONS may emerge as important cause of nosocomial infection.<o:p></o:p> Aims & Objectives: To detect prevalence of CONS and their ability to form biofilm by using three different screening methods. Also to determine antimicrobial susceptibility pattern among these isolates and correlate antibiotic susceptibility pattern of CONS and biofilm production.<o:p></o:p> Material and Method: A total of 100 CONS isolated from various clinical samples were subjected to antibiotic susceptibility testing and screened for biofilm production by Microtitre plate method, Tube method and Congo red agar method.<o:p></o:p> Results: CONS were most commonly isolated from pus samples (39%). Staphylococcusepidermidis (62%) was most commonly isolated CONS species. Maximum number of CONS isolates were resistance to ampicillin (67%) followed by Cotrimoxazole (66%). Maximum biofilm production was detected by MTP method (63%), followed by Tube method (52%) and CRA method (16%). Maximum biofilm producers were isolated from surgical wounds (pus) (20%). The Biofilm producing CONS isolates showed higher antibiotic resistance than non-biofilm producers.<o:p></o:p> Conclusion: The virulence of CONS is directly related to its capability to formbiofilms. There is association between biofilm production with persistent infection and antibiotic failure. Hence, in small microbiological laboratories where PCR cannot be done, Microtitre plate method can be recommended which is simple and cost effective. Due to biofilm formation very soon CONS may emerge as one of the leading nosocomial pathogen and should no longer be considered as a harmless commensal.<o:p></o:p>

Keywords :

CONS- Coagulase-negative staphylococci EPS-Extracellular polymeric substances PIA-Polysaccharide intercellular adhesion MTP-Microtitre plate method TM-Tube method CRA-Congo Red Agar method PBS-Phosphate buffer saline

Article:

Download PDF Journal DOI : 10.15373/2249555X

Cite This Article:

Dr. Anita E. Chand, Dr. Danish, Dr. Pradhuman Singh Chauhan, Phenotypic characterization and biofilm detection of Coagulase negative Staphylococci isolated from clinical specimens in Kota region, GLOBAL JOURNAL FOR RESEARCH ANALYSIS : Volume-7 | Issue-5 | May-2018