Volume : 7, Issue : 6, June - 2018

Screening of Biofilm Producing Bacteria from Various Clinical Samples

Malasane P. R. , Barate D. L.

Abstract :

<p class="MsoNormal" style="margin-top:6.0pt;margin-right:0in;margin-bottom:6.0pt;&#10;margin-left:0in;text-align:justify;line-height:150%"><span style="font-size:&#10;12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Biofilm is a microbial community which is embedded in extra cellular matrix.<span style="mso-spacerun:yes">&nbsp; </span>The ability of microorganism to develop biofilm is an important virulence factor and they are main cause of many chronic infections.<span style="mso-spacerun:yes">&nbsp; </span>They are responsible for the emergence of multi drug resistant strains resulting in treatment failure.<span style="mso-spacerun:yes">&nbsp; </span>In the present study a total of 50 bacteria were isolated from various clinical samples viz. blood, urine and pus.<span style="mso-spacerun:yes">&nbsp; </span>Out of 50 isolates 35 isolates were found to be biofilm producers after screening by Tube method (TM) and Congo Red Agar (CRA) method.<span style="mso-spacerun:yes">&nbsp; </span>These isolates were identified by standard conventional methods, which reveals that the biofilm producers were belongs to <i style="mso-bidi-font-style:normal">Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae </i>and<i style="mso-bidi-font-style:&#10;normal"> Pseudomonas aeruginosa</i>.<o:p></o:p></span></p>