Volume : 7, Issue : 10, October - 2018

ASSOCIATION BETWEEN VEGF +936C>T GENE POLYMORPHISM WITH GASTRIC PREMALIGNANT LESIONS AND VEGF LEVELS IN HELICOBACTER PYLORI PATIENTS

Gontar Alamsyah Siregar, Zaimah Z Tala, Ginanda Putra Siregar

Abstract :

<p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify;line-height:200%"><span lang="IN" style="font-size:12.0pt;line-height:&#10;200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;&#10;mso-fareast-language:IN">The objective of this study was t</span><span style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black;mso-ansi-language:EN-US;mso-bidi-font-weight:bold">o evaluate the </span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-bidi-font-style:italic">association</span><span style="font-size:12.0pt;&#10;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-ansi-language:EN-US;&#10;mso-bidi-font-style:italic"> between VEGF +936C&gt;T gene polymorphism</span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-bidi-font-style:italic">with </span><span style="font-size:12.0pt;&#10;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-ansi-language:EN-US;&#10;mso-bidi-font-style:italic">gastric premalignant lesion</span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-bidi-font-style:italic">s as well as the </span><span style="font-size:&#10;12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-ansi-language:&#10;EN-US;mso-bidi-font-style:italic">serum </span><span lang="IN" style="font-size:&#10;12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-bidi-font-style:&#10;italic">VEGF</span><span style="font-size:12.0pt;line-height:200%;font-family:&#10;&quot;Times New Roman&quot;,&quot;serif&quot;;mso-ansi-language:EN-US;mso-bidi-font-style:italic">levels</span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-bidi-font-style:italic">.</span><span lang="IN" style="font-size:12.0pt;&#10;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&#10;&quot;Times New Roman&quot;;mso-fareast-language:IN">This</span><span style="font-size:&#10;12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black;&#10;mso-ansi-language:EN-US"> cross-sectional study included 80 <i style="mso-bidi-font-style:normal">H. pylori</i> patients </span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black">atHaji </span><span style="font-size:12.0pt;line-height:200%;&#10;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black;mso-ansi-language:EN-US">Adam Malik General Hospital</span><span lang="IN" style="font-size:12.0pt;line-height:&#10;200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black">and Permata Bunda General Hospital</span><span style="font-size:12.0pt;line-height:200%;&#10;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black;mso-ansi-language:EN-US">, Medan, Indonesia. </span><span lang="IN" style="font-size:12.0pt;line-height:&#10;200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black">D</span><span style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black;mso-ansi-language:EN-US">iagnosis<span style="mso-bidi-font-style:&#10;italic">of <i>H.pylori</i> infection was made if </span></span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black;mso-bidi-font-style:italic">positive results of </span>^{<span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black">14</span>}<span lang="IN" style="font-size:12.0pt;line-height:&#10;200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black">C-UBT</span><span style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black;mso-ansi-language:EN-US"> and/orCLO </span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black">test</span><span style="font-size:12.0pt;line-height:200%;&#10;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black;mso-ansi-language:EN-US"> were found. If anyfindingssuch as</span><span style="font-size:12.0pt;&#10;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-ansi-language:EN-US">chronic atrophic gastritis, intestinal metaplasia, or dysplasia were present in microscopic evaluation, a<span style="color:black">diagnosis of gastric premalignant lesion was made</span>.<span style="color:black"> Real time polymerase chain reaction</span></span><span lang="IN" style="font-size:12.0pt;line-height:&#10;200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black"> (RT-PCR)</span><span style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black;mso-ansi-language:EN-US"> was used to examine <span style="mso-bidi-font-style:italic">VEGF +936C&gt;T gene polymorphism</span>. Level of circulating VEGF was determined using serum samples. </span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black">Data</span><span style="font-size:12.0pt;line-height:200%;&#10;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black;mso-ansi-language:EN-US">were collected and analyzed using SPSS version 22</span><span style="font-size:12.0pt;&#10;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-ansi-language:EN-US">.There was no significant association between VEGF +936C&gt;T polymorphism and gastric premalignant lesions (p&gt;0.05).Difference in serum VEGF levels between genotypes and alleles </span><span lang="IN" style="font-size:12.0pt;line-height:&#10;200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">of VEGF +936 C&gt;T polymorphism </span><span style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;mso-ansi-language:EN-US">were not significant (p=0.179 and p=0.076 respectively). </span><span lang="SV" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black;mso-ansi-language:SV">VEGF +936C&gt;T</span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black"> polymorphism</span><span lang="SV" style="font-size:12.0pt;&#10;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black;mso-ansi-language:&#10;SV"> was not associated with an increased risk of gastric premalignant transformation </span><span lang="IN" style="font-size:12.0pt;line-height:200%;&#10;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black">and </span><span style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black;mso-ansi-language:EN-US">serum</span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black"> VEG</span><span lang="SV" style="font-size:12.0pt;line-height:200%;&#10;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black;mso-ansi-language:SV">F</span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black"> levels</span><span lang="SV" style="font-size:12.0pt;line-height:&#10;200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black;mso-ansi-language:SV"> in <i style="mso-bidi-font-style:normal">H.pylori</i></span><span lang="IN" style="font-size:12.0pt;line-height:200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;&#10;color:black">p</span><span lang="SV" style="font-size:12.0pt;line-height:200%;&#10;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;color:black;mso-ansi-language:SV">atients.</span><b style="mso-bidi-font-weight:normal"><span style="font-size:12.0pt;line-height:&#10;200%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;&#10;mso-ansi-language:EN-US;mso-fareast-language:IN"><o:p></o:p></span></b></p>