Volume : 3, Issue : 6, June - 2014

To Evaluate the Effect of Aging on Complete Blood Count Parameters Done by Automated Cbc Analyzer

Dr Yogendra Madan, Dr R Varadharaja Perumal, Dr Vani Santosh Joshi

Abstract :

Introduction: Complete blood count (CBC) is one of the most common and conventional blood test done as daily routine. However the results of this test are affected by different factors such as, the duration of incubation,  Aim: This survey was to evaluate the effect of time of incubation on CBC. Method: In a cross–sectional study of 400 randomly selected adequate blood samples collected in k2EDTA vacutainer were analyzed by fully automated three part hematology analyzer at time interval of 20 min, then after 2, 8, 24, 72 hours at room temperature. Result: MCV, MPV, Hct and RDW increases significantly with passage of time while MCHC decreases. WBC and platelet count were reliable upto 24 hour at room temperature. MCH, RBC and hemoglobin values were stable parameter for more than 72 hours. Conclusion: It is better to do the CBC test after blood taking as soon as possible. Various parameter change significantly with passage of time. RBC and hemoglobin values were stable if test is carried out following a delay