Volume : 4, Issue : 1, January - 2015

Genistein substantiate and fortifyoxidant potential of a cell by interacting with Bcl-2: an anti-apoptotic protein in rat model.

Humairafarooqi, Kailash Singh, Ajaz Ahmad Ganai

Abstract :

<p>Genistein, an isoflavonoid isknown for its antioxidant property. It is known that the presence of Bcl&ndash;2 is important for the< /> antioxidant action of genistein. The possible mechanism of action of the genistein is by its interaction with Bcl&ndash;2, widely< /> known for its characteristic to transport the GSH from the cytosol to the nucleus,protecting cells against mitochondrial< /> oxidative stress and subsequent apoptosis.Here, we have validated the antioxidative effect of genistein on rat model (rattusnorvegicus) where< /> oxidative stress was induced by D&ndash;GalN treatment. Genistein significantly attenuated the effects of D&ndash;GalN and levels of marker enzymes like AST,< /> ALT and BCL&ndash;2 which were reestablished in the animals pretreated with it. We also introduce a computationally comprehensiveprotein 3&ndash;D model< /> of Bcl&ndash;2 of rattusnorvegicus by using CPH model, SAVES and SWISS&ndash;MODEL workspace (http://www.cbs.dtu.dk/services/CPHmodels,http://< /> nihserver.mbi.ucla.edu/SAVES/,http://swissmodel.expasy.org/qmean/cgi/index.cgi)and it was validated withvarious computational tools like< /> ERRAT, PROCHECK, VERIFY 3D etc. Wealso present docking study to check the interaction between the Bcl&ndash;2 and genistein with the help of web< /> based serverpatch dock, an online tool for Ligand Protein Docking (http://bioinfo3d.cs.tau.ac.il/PatchDock).Docking score between Bcl&ndash;2 and< /> genistein was found to be165.38 kcal/mol, which validates the interaction between them. Therefore, it is proposed that the genistein works on to< /> the Bcl&ndash;2 to increase the uptake of GSH from cytoplasm to nucleus.</p>