Volume : 4, Issue : 4, April - 2015

INDIRECT, DIRECT AND SECONDARY SOMATIC EMBRYOGENESIS IN EMBLICA OFFICINALIS

Priyanka, Upendra Kumar, K. P. Singh

Abstract :

<p>&lt;div&gt;A continuously growing callus from cotyledonary leaves was achieved in vitro on MS medium. After 2, 3 sub cultures&amp;nbsp;&lt;/div&gt; &lt;div&gt;on the same medium the callus became emyogenic and numerous emyos of different stages of development were&amp;nbsp;&lt;/div&gt; &lt;div&gt;observed on the surface of the callus. Direct somatic emyogenesis from cotyledons, and callus mediated (indirect)&amp;nbsp;&lt;/div&gt; &lt;div&gt;emyogenesis was observed when mature cotyledons were cultured on BMS medium supplemented with different concentrations ofauxins&amp;nbsp;&lt;/div&gt; &lt;div&gt;and cytokinins. 2,4&amp;ndash;D alone, as well in combination of Kn led to somatic emyogenesis. BMS supplemented with IAA, IBA, BAP and Kn led to&amp;nbsp;&lt;/div&gt; &lt;div&gt;direct emyogenesis without callus formation. IAA and IBA on sub culture give rise to secondary emyos from culture raised emyos. When&amp;nbsp;&lt;/div&gt; &lt;div&gt;transferred on MS without hormone these emyos give rise to complete plants Plantlets were regenerated from the emyos 2 months after their&amp;nbsp;&lt;/div&gt; &lt;p&gt;transfer to the germination medium without any growth regulators but with the addition of 30 g/l sucrose.&amp;nbsp;&lt;/p&gt;</p>