Volume : 6, Issue : 1, January - 2017

SIGNIFICANCE OF IMMUNOHISTOCHEMISTRY IN DETECTION OF H.PYLORI IN NASAL POLYPS.

Dr Sushma S Nigudgi, Dr Anuradha G Patil

Abstract :

<p>&nbsp;<b style="text-align: justify;"><u><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,serif">BACKGROUND</span></u></b><span style="text-align: justify; font-size: 12pt; font-family: &quot;Times New Roman&quot;, serif;">- Helicobacter pylori is recognized as an important human pathogen associated with peptic ulcer disease, gastric cancer, and has high prevalence of infection worldwide. Recently this organism was detected in tonsillar and adenoid tissue, as well as in nasal mucosa of patients with chronic rhinosinusitis and nasal polyposis.</span></p> <p class="MsoNormal" style="margin-bottom:.5pt;text-align:justify;text-justify:&#10;inter-ideograph;line-height:normal"><b><u><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,serif">AIM</span></u></b><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,serif">- 1)To study the presence of H.pylori in nasal polyps.<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:.5pt;text-align:justify;text-justify:&#10;inter-ideograph;line-height:normal"><span style="font-size:12.0pt;font-family:&#10;&quot;Times New Roman&quot;,serif">2) To study and compare the role of immunohistochemistry in the detection of H.pylori.<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:.5pt;text-align:justify;text-justify:&#10;inter-ideograph;line-height:normal"><span style="font-size:12.0pt;font-family:&#10;&quot;Times New Roman&quot;,serif">METHODS - 30 nasal polypectomy specimens studied and compared to detect the presence of H.pylori by urease test (CLO), Giemsa stain and Immunohistochemistry using H.pylori antibody.<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:.5pt;text-align:justify;text-justify:&#10;inter-ideograph;line-height:normal"><b><u><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,serif">RESULTS</span></u></b><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,serif">- H-pylori was detected in nasal polyps by Urease test in 6.66% cases and by Giemsa stain in 16.66%, But by immunohistochemistry in 26.66% (8/30) cases significantly(P&lt; 0.05).The 6/8 IHC positive cases were inflammatory polyps.<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:.5pt;text-align:justify;text-justify:&#10;inter-ideograph;line-height:normal"><b><u><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,serif">CONCLUSION</span></u></b><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,serif">- 1) In present study significant association of H.Pylori is detected pointing towards its role in etiopathogenesis of nasal polyps.<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:.5pt;text-align:justify;text-justify:&#10;inter-ideograph;line-height:normal"><span style="font-size:12.0pt;font-family:&#10;&quot;Times New Roman&quot;,serif">2)Treatment of H.Pylori may help in nasal polyposis especially in recurrence.<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:.5pt;text-align:justify;text-justify:&#10;inter-ideograph;line-height:normal"><span style="font-size:12.0pt;font-family:&#10;&quot;Times New Roman&quot;,serif">3) IHC is significantly better method for detection of H.Pylori than urease test(P&lt;0.05) and can be considered cost effective for detection of H.Pylori in recurrent nasal polyposis.<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:.5pt;text-align:justify;text-justify:&#10;inter-ideograph;line-height:normal"><span style="font-size:12.0pt;font-family:&#10;&quot;Times New Roman&quot;,serif">However larger epidemiological studies would be needed<o:p></o:p></span></p>