Volume : 6, Issue : 2, February - 2017

PLASMA VITAMIN D LEVEL AND VITAMIN D RECEPTOR GENETIC POLYMORPHISM OF SPINAL TUBERCULOSIS SUSCEPTIBILITY IN WEST JAVA POPULATION, INDONESIA: DOES IT CORRELATED?

Ahmad Ramdan, Ida Parwati, Budi Setiabudiawan, Bambang Tiksnadi

Abstract :

<p>&nbsp;<b style="text-align: justify;"><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">To explore the potential role of plasma vitamin D level and VDR gene polymorphisms in susceptibility to tuberculosis (TB) in the West Java population, Indonesia. A case-control study was performed on the West Java population, Indonesia. Plasma Vitamin D levels were analyzes using ELISA. Genetic polymorphisms of VDR gene (<i>T</i>aqI, <i>F</i>okI, <i>B</i>smI, <i>A</i>paI) were analyzed using PCR-RFLP and sequencing analysis in 53 Spinal TB patients, 53 Pulmonary TB patients and 53 healthy controls. T</span></b><b style="text-align: justify;"><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">here was significant difference (p&lt;0.01) of plasma vitamin D between all group. Genotype analysis of </span></b><b style="text-align: justify;"><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">gene </span></b><b style="text-align: justify;"><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">polymorphism</span></b><b style="text-align: justify;"><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;"> VDR&nbsp; </span></b><b style="text-align: justify;"><i><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">F</span></i></b><b style="text-align: justify;"><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">ok1, <i>B</i>sml, <i>A</i>pal and <i>T</i>aql shows no significant results in all group (p&gt;0.01). Combination of vitamin D deficiency and </span></b><b style="text-align: justify;"><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">gene </span></b><b style="text-align: justify;"><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">polymorphism <i>F</i>olk in FF genotype shows significant result to</span></b><b style="text-align: justify;"><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;"> the susceptibility</span></b><b style="text-align: justify;"><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;"> of spinal tuberculosis (p&lt;0.01).</span></b><b style="text-align: justify;"><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;"> </span></b><b style="text-align: justify;"><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">T</span></b><b style="text-align: justify;"><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">he combination of vitamin D deficiency and </span></b><b style="text-align: justify;"><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">gene </span></b><b style="text-align: justify;"><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">polymorphism <i>F</i>olk in FF genotype were contributing factor</span></b><b style="text-align: justify;"><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">s</span></b><b style="text-align: justify;"><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;"> to the sus</span></b><b style="text-align: justify;"><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">c</span></b><b style="text-align: justify;"><span lang="IN" style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">eptibility of spinal tuberculosis in the West Java population, Indonesia</span></b><b style="text-align: justify;"><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;">.</span></b></p> <p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify"><b><span style="font-size: 10pt; line-height: 115%; font-family: &quot;Times New Roman&quot;, serif;"><o:p></o:p></span></b></p>