Volume : 6, Issue : 7, July - 2017

Association of CXCR1 expression with Histological grade and Clinical stage of Oral Squamous Cell Carcinoma.

Dr Basavaraj Kallapur, Dr Wafa Awedat, Dr. Omneya Mahomoud

Abstract :

<p>&nbsp;<b style="text-align: justify;"><span style="font-size:10.0pt;line-height:170%;font-family:&quot;Times New Roman&quot;,serif">Background:</span></b><span style="text-align: justify; font-size: 10pt; line-height: 170%; font-family: &quot;Times New Roman&quot;, serif;">. Interleukin-8 (IL-8) is a pro-inflammatory cytokine which exerts its effects via binding to CXCR1 and is known to promote angiogenesis. CXCR1 represent potential prognostic biomarkers and therapeutic target.</span></p> <p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify;line-height:170%;mso-layout-grid-align:none;text-autospace:none"><b><span style="font-size:10.0pt;line-height:&#10;170%;font-family:&quot;Times New Roman&quot;,serif">Aim of the study:</span></b><span style="font-size:10.0pt;line-height:170%;font-family:&quot;Sylfaen&quot;,serif;&#10;mso-bidi-font-family:&quot;Times New Roman&quot;"> </span><span style="font-size:10.0pt;&#10;line-height:170%;font-family:&quot;Times New Roman&quot;,serif;mso-ascii-theme-font:major-bidi;&#10;mso-hansi-theme-font:major-bidi;mso-bidi-font-family:Arial;mso-bidi-theme-font:&#10;minor-bidi">To evaluate the expression of CXCR1 in OSCC and control tissue&nbsp; as well&nbsp; as&nbsp; correlate&nbsp; the expression of the CXCR1to the&nbsp; clinicopathologic parameters of OSCC .<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify;line-height:170%"><b><span style="font-size:10.0pt;line-height:170%;font-family:&quot;Times New Roman&quot;,serif">Material and Methods:</span></b><span style="font-size:10.0pt;line-height:170%"> </span><span style="font-size:10.0pt;line-height:170%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-ascii-theme-font:major-bidi;mso-hansi-theme-font:major-bidi;mso-bidi-font-family:&#10;Arial;mso-bidi-theme-font:minor-bidi">Immunohistochemical analysis&nbsp; of 22 cases OSCC and 7 control cases sections&nbsp; stained by&nbsp; anti-CXCR1antibody.</span><span style="font-size:10.0pt;line-height:170%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-ascii-theme-font:major-bidi;mso-fareast-font-family:&quot;Times New Roman&quot;;&#10;mso-fareast-theme-font:minor-fareast;mso-hansi-theme-font:major-bidi;&#10;mso-bidi-font-family:Arial;mso-bidi-theme-font:minor-bidi;mso-bidi-language:&#10;AR-EG"> Immunohistochemical staining will be performed using a Labeled Strept-Avidin Biotin complex method (LSAB).<o:p></o:p></span></p> <p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify;line-height:150%"><b><i><span style="font-size:10.0pt;line-height:150%;&#10;font-family:&quot;Times New Roman&quot;,serif">Results:</span></i></b><span style="font-size:10.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-ascii-theme-font:major-bidi;mso-hansi-theme-font:major-bidi;mso-bidi-font-family:&#10;Arial;mso-bidi-theme-font:minor-bidi">Expression was recognized in all cases of&nbsp; OSCC and </span><span style="font-size:8.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-ascii-theme-font:major-bidi;mso-hansi-theme-font:major-bidi;mso-bidi-font-family:&#10;Arial;mso-bidi-theme-font:minor-bidi">normal</span><span style="font-size:10.0pt;&#10;line-height:150%;font-family:&quot;Times New Roman&quot;,serif;mso-ascii-theme-font:major-bidi;&#10;mso-hansi-theme-font:major-bidi;mso-bidi-font-family:Arial;mso-bidi-theme-font:&#10;minor-bidi"> &nbsp;control cases and </span><span style="font-size:10.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-ascii-theme-font:major-bidi;mso-hansi-theme-font:major-bidi;mso-bidi-theme-font:&#10;major-bidi;letter-spacing:-.1pt">the pattern of expressed in both the cytoplasm and &nbsp;nucleus of the malignant epithelial cells (total cell reactivity)</span><span style="font-size:10.0pt;line-height:&#10;150%;font-family:&quot;Times New Roman&quot;,serif;mso-ascii-theme-font:major-bidi;&#10;mso-hansi-theme-font:major-bidi;mso-bidi-font-family:Arial;mso-bidi-theme-font:&#10;minor-bidi">,</span><span style="font-size:10.0pt;line-height:150%;font-family:&#10;&quot;Times New Roman&quot;,serif;mso-ascii-theme-font:major-bidi;mso-hansi-theme-font:&#10;major-bidi;mso-bidi-theme-font:major-bidi"> as well as high expression was noticed in low histological grade and clinical stage malignancy, whereas decrease of expression was detected in high grade and stage malignancy.</span><b><i><span style="font-size:10.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,serif"><o:p></o:p></span></i></b></p> <p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:&#10;justify;line-height:150%;mso-layout-grid-align:none;text-autospace:none"><b><i><span style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-ascii-theme-font:major-bidi;mso-hansi-theme-font:major-bidi;mso-bidi-theme-font:&#10;major-bidi">Conclusions:</span></i></b><span style="font-size:12.0pt;&#10;line-height:150%;font-family:&quot;Times New Roman&quot;,serif;mso-ascii-theme-font:major-bidi;&#10;mso-hansi-theme-font:major-bidi;mso-bidi-theme-font:major-bidi"> </span><span style="font-size: 10pt; line-height: 150%; font-family: &quot;Times New Roman&quot;, serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">the results show an association between </span><span style="font-size:10.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,serif;&#10;mso-ascii-theme-font:major-bidi;mso-hansi-theme-font:major-bidi;mso-bidi-theme-font:&#10;major-bidi;letter-spacing:-.1pt">CXCR1</span><span style="font-size: 10pt; line-height: 150%; font-family: &quot;Times New Roman&quot;, serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">and the </span><span style="font-size:10.0pt;line-height:&#10;150%;font-family:&quot;Times New Roman&quot;,serif;mso-ascii-theme-font:major-bidi;&#10;mso-hansi-theme-font:major-bidi;mso-bidi-theme-font:major-bidi">clinicopathologic parameters<span style="background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">, and </span><span style="letter-spacing:-.1pt">considered as prognostic marker in OSCC</span></span><span style="font-size: 12pt; line-height: 150%; font-family: &quot;Times New Roman&quot;, serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">.<o:p></o:p></span></p>