Volume : 2, Issue : 7, July - 2013

Simultaneous estimation of quercetin and rutin in ethanolic extract of Catharanthus roseus. Linn leaves by HPTLC method

A. Srinivasa Rao, Mohammed Fazil Ahmed

Abstract :

The aim of the study is to estimation the two biologically active flavonoidal compounds, quercetin and rutin, in ethanolic leaves extract of Catharanthus roseus. Linn leaves by using high–performance thin–layer chromatography (HPTLC). Pre coated silica gel 60F254 used as stationary phase and Toluene: Ethyl Acetate: methanol in ratio of 5: 3: 2 are used as mobile phase. Densitometric determination and quantification of these compounds was carried out at 254 nm.The standard Rf values of rutin and quercetin are 0.17 and 0.65 respectively. The total peak areas of the standards, quercetin and rutin were compared and the corresponding peak areas of extracts were estimated to be 1914.15 and 552.24 respectively. This HPTLC method was found to be simple and convenient for rapid screening of active compounds and quantification of the investigated flavonoids in Catharanthus roseus. Linn.